Search results for "Tissue culture"

Effects of peroxidizing herbicides on protoporphyrin IX levels in non-chlorophyllous soybean cell culture

1990

Abstract The mode of action of 16 peroxidizing herbicides belonging to four different families (diphenyl ethers, oxadiazon, pyridine derivatives, and pyrazole derivatives) has been studied in nonchlorophyllous soybean cell cultures. Whenever possible, we have compared active and inactive compounds. Phytotoxic effects were estimated on the basis of growth inhibition, either in the dark or in the light. Protoporphyrin IX accumulations were estimated in dark-treated samples, using a simple methodology. In all cases, we have found a positive correlation between cellular damages and protoporphyrin IX accumulations. The results provide further evidences in favor of the light-dependent activity of…

Stomach signet-ring cancer cell line (MZ-STO-1), established in tissue culture: Morphological characterization and antigenic profile

1986

Isolation and culture of grape vine cv. Chardonnay leaf protoplasts

1990

Experimental conditions were established that resulted in high yields and good viability of the protoplasts obtained from leaves of Vitis vinifera, cv. Chardonnay regenerated in vitro by somatic embryogenesis. The effect of factors of the culture medium and various environmental conditions upon the frequency of cell division has been examined, and a method of culture is described by which protoplasts were induced to begin division. Most protoplasts obtained in this way regenerated cell walls within the first few days and cell division occurred after 10 days of culture in a liquid medium. Some cells have divided two or even three times. Nevertheless, the cells did not continue dividing beyon…

Microspore embryogenesis inCitrusand other fruit crops

2017

Conventional methods, involving several generations of selfing, are not applicable to produce homozygous lines in Citrus as well as in the other fruit crops, due to the high heterozygosity of the genomes, the long duration of the generation cycle, the large size, and, often, the self-incompatibility. For this reason, there is no different way to obtain homozygosity in this kind of plants than “gametic embryogenesis” that allows the development of haploids (Hs, plants with gametophytic chromosome number) and doubled haploids (DHs, haploids that have undergone chromosome duplication) from heterozygous parents in a single step. Therefore, gametic embryogenesis is increasingly object of res…

Relation of lenacil metabolism with growth inhibition of acer pseudoplatanus cell suspension

1983

Abstract The action of lenacil, a herbicide which inhibits photosynthesis, was studied on Acer pseudoplatanus cell suspensions. The compound was quickly and thoroughly metabolized by cells into two major chloroform-extractable metabolites, but cell growth was temporarily inhibited while some cells were killed. As cell suspensions were non-photosynthetic, the data suggest that lenacil has site(s) of action other than that of photosynthesis. However, as the effects on photosynthesis occur at much lower concentrations (Hilton et al., Weeds, 12 (1964) 129), the effects on cell growth may be considered as secondary.

Low level 809-nm diode laser-induced in vitro stimulation of the proliferation of human gingival fibroblasts

2002

Background and Objective The authors investigated the effects of low level laser irradiation on the proliferation rate of human gingival fibroblasts (HGF) in vitro. Study Design/Materials and Methods HGF were obtained from gingival connective tissue explants and cultured under standard conditions. 110 cell cultures in their logarithmic growth phase were spread on 96-well tissue culture plates and were irradiated at energy fluences of 1.96–7.84 J/cm2. Another 110 cultures served as control. An 809-nm semiconductor laser operated at a power output of 10 mW in the cw-mode was used. The time of exposure varied between 75 and 300 seconds. Laser treatment was performed alternatively once, twice, …

First stages of microsporere programming to embryogenesis through anther culture in Prunus armeniaca L.

2011

6 páginas, 3 figuras, 2 tablas -- PAGS nros. 152-157

Anther culture in Citrus clementina: a way to regenerate tri-haploids

2005

Abstract. Regenerants from anther culture of Citrus clementina Hort. ex Tan. cvv. Nules, SRA 63, and Monreal were obtained in different experiments from 1994 to 2002. Genetic analysis of 37 such regenerants was carried out using 4 microsatellite markers that were heterozygous in the parental genotypes. The results showed that in all cases but one the regenerants carried only one or the other allele of the parental genotype, and were therefore homozygous and produced through a process of gametophytic embryogenesis. Ploidy analysis by flow cytometry of 94 regenerants showed that as many as 82% of them were tri-haploids, rather than haploids or doubled-haploids as expected, with other ploidy …

Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification

2011

Objective To investigate four different protocols for cryopreservation of the whole rat ovary with intact vasculature to evaluate whether differences exist in post-thawing viability of the ovary after either vitrification or slow freezing. Design Experimental study. Setting Obstetrics and gynecology department. Animal(s) Immature Sprague-Dawley female rats. Intervention(s) Ovaries were isolated with the vascular tree intact up to the bifurcation of the abdominal aorta and were subsequently cannulated. The ovaries were flushed with increasing concentrations of the cryoprotectant dimethyl sulfoxide (DMSO) to either 1.5 or 7 M. The ovaries underwent cryopreservation by vitrification or passive…

Low-level 809 nm GaAlAs laser irradiation increases the proliferation rate of human laryngeal carcinoma cells in vitro

2002

The aim of the study was to investigate the effect of low-level 809 nm laser irradiation on the proliferation rate of human larynx carcinoma cells in vitro. Epithelial tumor cells were obtained from a laryngeal carcinoma and cultured under standard conditions. For laser treatment the cells were spread on 96-well tissue culture plates. Sixty-six cell cultures were irradiated with an 809 nm GaAlAs laser. Another 66 served as controls. Power output was 10 mW(cw) and the time of exposure 75–300 s per well, corresponding to an energy fluence of 1.96–7.84 J/cm2. Subsequent to laser treatment, the cultures were incubated for 72 h. The proliferation rate was determined by means of fluorescence acti…